Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot
Figure 2
SDS-PAGE analysis on purified rGRA5 protein. (a) Coomassie blue stained. Lane 2: purified pRSET B. Lane 3: purified rGRA5 and (b) western blot probed with toxoplasmosis-infected patient’s serum. Lane 2: purified rGRA5. Lane 3: purified pRSET B. Lane 1 (panel a and panel b) is the prestained broad range protein marker. The 20 kDa purified rGRA5 was detected (arrow).
