Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot
Figure 3
Western blots of purified rGRA5 protein with sera of toxoplasmosis and toxoplasmosis-negative patients. Lane 1 (panels a–c): the prestained broad range protein marker. Lanes 2 (a) to 6 (a): results of 5 sera from chronic-profile patients (Group 3: IgG +ve, IgM −ve). Lanes 2 (b) to 6 (b): results of 5 sera from acute-profile patients (Group 2: IgG +ve, IgM +ve). Lanes 2 (c) to 6 (c): results of 5 sera from toxoplasmosis-negative patients (Group 4: IgG −ve, IgM −ve). The 20 kDa purified rGRA5 was detected by toxoplasmosis-positive sera (arrow).
