Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot

Table 1

Immunoreactivities (sensitivity and specificity) of the rGRA5 antigen to serum samples from toxoplasmosis-positive and toxoplasmosis-negative patients.

Serum samples group	Number of human serum samples	Immunoreactivities
		Positive		Negative
		Number	%	Number	%
1 (Early acute: IgG−ve, IgM+ve)	44	0	0	44	100
2 (Acute: IgG+ve, IgM+ve)	47	22	46.8	25	53.2
3 (Chronic: IgG+ve, IgM−ve)	85	52	61.2	33	38.8
4 (Toxoplasmosis-negative: IgG−ve, IgM−ve)	24	0	0	24	100
Other infections	12	1	8.3	11	91.7
Amoebiasis	3	0	0	3	100
Cysticercosis	3	0	0	3	100
Filariasis	3	0	0	3	100
Toxocariasis	3	1*	33.3	2	66.7

∗One out of three toxocariasis-positive sera samples reacted with the rGRA5 antigen. This particular toxocariasis-positive serum sample was shown to be IgG positive for toxoplasmosis based on the commercial kits.