Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

Figure 1

Cryostat cross sections through naïve rat sciatic nerve (a), after sham operation (b), and distal to crush for 7 d (c) immunostained for IL-1β revealed robust increase of immunofluorescence distal to nerve injury. Increased IL-1β immunofluorescence was found dominantly in Schwann cells detected by colocalization with GFAP (arrowheads, (d)–(f)). Growing axons and sprouts immunostained with GAP43 (arrows) were found in close contact with Schwann cells decorated by IL-1β immunostaining (arrowheads) ((g)–(i)). Scale bars for (a)–(c) = 20 μm, for (d)–(i) = 5 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)