Efficient Expression of Acetylcholine-Binding Protein from Aplysia californica in Bac-to-Bac System
Figure 3
Comparison of two methods used for testing protein expression from different cell densities. (a): Test protein expression level by gel filtration chromatography method different cell densities expression level (from high to low: A, 2 × 106 cells/mL; B, 4 × 106 cells/mL; C, 1 × 106 cells/mL). (b): Test protein expression level by SDS-PAGE method from different cell densities (lane A, 2 × 106 cells/mL; lane B, 4 × 106 cells/mL; lane C, 1 × 106 cells/mL. RA, RB, and RC indicated reduced stated of Ac-AChBP expression samples, respectively, from lanes A, B, and C), the result of test expression level method analysis was the same as gel filtration chromatography.
