Does Platelet-Rich Plasma Freeze-Thawing Influence Growth Factor Release and Their Effects on Chondrocytes and Synoviocytes?

Table 2

Soluble factor concentrations in fresh (PRP) and frozen PRP 1 h and 7 days after activation. Concentrations are expressed as pg/mL and reported as median values and (interquartile ranges).

Soluble factors	Preparations	Incubation time		𝑃 value
		1 hour	7 days
IL-1β	PRP	1.015 (0.83–5.41)	70.11 (56.81–233.35)	𝑃<0.01
	Frozen PRP	1.22 (0.72–2.32)	14.43 (1.11–179.20)	NS
	𝑃 value	NS	NS
TGF-β1	PRP	107861.6 (81652–127793.0)	103553.4 (64935.69–1341400.0)	NS
	Frozen PRP	33849.8 (23339.08–54974.0)	52511.5 (30092.61–201434.0)	NS
	𝑃 value	𝑃<0.005	𝑃<0.01
PDGF AB/BB	PRP	27714.68 (18591.50–35850.24)	31670.63 (18617.58–80462.27)	NS
	Frozen PRP	17388.90 (8648.29–29500.03)	6035.78 (4691.41–37053.02)	NS
	𝑃 value	𝑃<0.01	𝑃<0.01
VEGF	PRP	157.94 (62.3–238.52)	226.79 (145.82–743.31)	NS
	Frozen PRP	147.78 (9.39–209.94)	204.10 (136.85–632.23)	NS
	𝑃 value	NS	NS
HGF	PRP	247.20 (148.75–305.97)	380.89 (370.58–493.17)	𝑃<0.01
	Frozen PRP	253.68 (109.87–283.97)	212.77 (149.44–261.94)	NS
	𝑃 value	NS	𝑃<0.01