Diazepam Potentiates the Antidiabetic, Antistress and Anxiolytic Activities of Metformin in Type-2 Diabetes Mellitus with Cooccurring Stress in Experimental Animals

Figure 4

The effect of MET, DZ, and their combination on DMS-induced changes in the level of expression of phospho-Akt^ser473 (p-Akt^ser473) and total Akt in the liver tissues. The blots are representative of p-Akt^ser473 and total Akt (a) in the liver tissues. The results in the histogram are expressed as a ratio of relative intensity of levels of protein expression of either p-Akt^ser473 or total Akt to 𝛽-actin and a ratio of relative intensity of level of expression of p-Akt^ser473 to total Akt. All values are mean ± SEM of three separate sets of independent experiments. ^a𝑃<0.05 compared to control, ^b𝑃<0.05 compared to DMS, ^c𝑃<0.05 compared to DMS+MET, and ^d𝑃<0.05 compared to DMS+DZ (one-way ANOVA followed by Student Newmann-Keuls test).

(a)

(b)

(c)

(d)