Diazepam Potentiates the Antidiabetic, Antistress and Anxiolytic Activities of Metformin in Type-2 Diabetes Mellitus with Cooccurring Stress in Experimental Animals

Table 3

Effect of metformin, diazepam, or their combination on plasma glucose, triglyceride, and corticosterone levels in cooccurring T2DM and RS exposed rats.

Groups	D-1	D-3	D-7	D-13
Plasma glucose (mg/dL)
Control	76.3±1.0	75.8±0.8	75.5±0.6	75.1±0.8
DMS	76.0±2.8	256.3±3.0^a	568.8±5.8^a	577.6±2.5^a
DMS + MET	74.5±2.7	261.1±2.5^a	572.9±5.1^a	425.8±4.7^a,b
DMS + DZ	75.3±1.7	254.3±2.7^a	566.2±4.8^a	574.2±1.8^a,c
DMS + MET + DZ	74.9±2.6	264.6±1.3^a	574.1±0.7^a	212.5±5.2^a,b,c,d
Plasma triglyceride (mg/dL)
Control	85.9±1.5	87.4±1.6	87.3±1.6	87.1±1.5
DMS	90.5±1.2	195.2±4.1^a	471.0±9.8^a	483.3±10.1^a
DMS + MET	88.8±1.5	194.1±7.0^a	474.2±11.1^a	365.6±9.0^a,b
DMS + DZ	91.3±1.2	196.2±4.8^a	470.1±9.8^a	484.1±9.6^a,c
DMS + MET + DZ	91.7±1.2	200.0±5.7^a	484.9±12.6^a	146.0±7.1^a,b,c,d
Plasma corticosterone (μg/dL)
Control	15.8±0.4	16.3±0.4	15.9±0.4	16.1±0.5
DMS	15.9±0.3	34.4±1.5^a	187.7±5.3^a	191.5±8.8^a
DMS + MET	15.4±0.6	32.0±1.1^a	188.4±5.7^a	120.5±3.9^a,b
DMS + DZ	15.2±0.7	31.7±1.0^a	186.7±6.5^a	107.1±2.6^a,b,c
DMS + MET + DZ	15.4±0.7	31.3±1.0^a	191.1±6.0^a	77.6±5.5^a,b,c,d

All values are mean ± SEM (𝑛=6). ^a𝑃<0.05 compared to control, ^b𝑃<0.05 compared to DMS, ^c𝑃<0.05 compared to DMS + MET, and ^d𝑃<0.05 compared to DMS + DZ (repeated measures of two-way ANOVA followed by Bonferroni post hoc test).