Liposomal Formulation of Turmerone-Rich Hexane Fractions from Curcuma longa Enhances Their Antileishmanial Activity
Figure 3
Ultrastructure alterations induced by LipoRHIC in L. amazonensis promastigotes. ((a)-(b)) Sections of untreated promastigote forms showing the main structures observed under transmission electron microscopy. (a) Promastigote presenting typical elongated body. The nucleus is rounded and the mitochondrion is branched. White arrow shows acidocalcisome in the cytoplasm; (b) normal flagellum and flagellar pocket are observed in the anterior portion of the parasite. Mitochondrion containing the kinetoplast can also be noted. ((c)–(f)) Parasites treated for 24 h with MIC concentration (5.5 μg/mL) of LipoRHIC. (c) Parasite presenting rounded body and an abnormal membrane projection (black arrow); (d) detail of mitochondrion swelling and the presence of several vacuoles (); ((e)-(f)) complete intracellular disorganization although the nucleus membrane remained intact. (f) Autophagic structure (★) and electron-dense granules (black arrows). n: nucleus; m: mitochondrion; k: kinetoplast; f: flagellum; fp: flagellar pocket; g: lipid.
