Induction of Apoptosis in Human Multiple Myeloma Cell Lines by Ebselen via Enhancing the Endogenous Reactive Oxygen Species Production

Figure 5

Mitochondrial membrane potential of MM cells analyzed by laser confocal microscopy and FACS after being loaded with JC-1. (a) Fluorescence images of JC-1 Probe loaded cells were obtained under laser confocal microscopy ((A) DMSO, (B) Ebselen 40 μM, and (C) ebselen 40 μM + NAC 15 mM). The ratio of red (FL2-H) and green (FL1-H) mean fluorescence intensity obtained under FACS shows ebselen induced MMP lost of MM cells in a concentration (b) and time (c) manner. Each value represents the mean ± SD from three independent experiments. *Significant difference compared to the control group (^∗𝑃<0.05, ^∗∗𝑃<0.01, one-way ANOVA with Scheffe’s test).

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(b)

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