Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients
Expression of ERVWE1 and ERVW2 in chondrocytes from OA and non- or early OA patients. (a) PCR on cDNA prepared from cultured chondrocytes isolated from knees or hip from OA patients. Specific primers for the ERVWE1 and ERVWE2 env gene (encoding syncytin) were used. (b) RNA purified from nitrogen-crushed cartilage. (c) RNA was isolated from chondrocytes obtained from 6 OA patients, converted into cDNA, and amplified using specific primers complementary to the gag gene of HERVWE1 (left panel) and HERVWE2 (right panel) sequences. (d) Amplification of cDNA prepared from chondrocytes from non- and early OA patients. Lanes 1 and 11: 1 kb plus ladder; lanes 2–10: amplified cDNA. The arrow indicates the presence of the amplified 3,000 bp of the ERVWE1 env transcript.
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