Research Article

Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

Table 1

Virus-specific primers used in this study.

VirusGenBank acc. numberPrimer sequencesPosition

HERVWE1
 Envelope (=syncytin)NM_014590F: cat cga tag cac cca tca gat g 13–34
R: gag tga aat agc atg aaa aca g 3025–3004
 ERVWE1 gag AF156961F: tgt ccg ctg tgc tcc tga tc 1–20
R: ctg cgc cag tgt cca gga gac 1921–1901

HERVWE2
 EnvelopeAF127228,F: cca ata gcc aga cca tta tat ac 1–23
AF128229,R: tgg ggt tcc att tgt aag acc 1932–1912
 ERVWE2 gagAF123881F: cta gaa cgt att ctg gag aat tg 2–24
R: ggc tct caa tgg tca aac ata c 1509–1488

HHV-1GU734772F: tcc cca taa act ggg agt agc 55192–55212
R: cag aac tac agc gag ggc atc 55327–55307

HHV-6NC_000898F: gag tcc atg agt tag aag att 150874–150894
R: cta aat ttt cta cct ccg aaa tgt 152099–152076

HHV-4 (Epstein Barr)V01555External primers
 F: agg gat gcc tgg aca caa ga 48810–48829
 R: tgg tgc tgc tgg tgg tgg caa t 49406–49385
Nested primers
 F: tcttgatag gga tcc gct agg ata 48839–48862
 R: acc gtg gttbctg gac tat ctg gat49335–49311

HHV-5 (human cytomegalovirus)GQ222016External primers
 F: cag cac cat cct cct ctt cct ctg 3665–3688
 R: cca agc ggc ctc tga taa cca agc 4099–4076
Nested primers
 F: aga cac tgg ctc aga cct gac 3708–3728
 R: aga gtc tgc tct cct agt gtg 3989–3969

Hepatitis C virusAB691598External primers
 F: ggc gac act cca cca tgg atc ac16–38
 R: cat gtt gca cgg tct acg aga cc342–320
Nested primers
 F: ctg tga gga act tct gtc tt 43–62
 R: ctc gca agc acc cta tca gg 309–290

Human parvovirus B19NC_000883External primers
 F: aat aca ctg tgg ttt tat ggg ccg 1579–1602
 R: cca ttg ctg gtt ata acc aca ggt 1862–1839
Nested primers
 F: aat gaa aac ttt cca ttt aat gat gta g 1678–1705
 R: cta aaa tgg ctt ttg cag ctt cta c1780–1756

Rabbit endogenous retrovirus (HRV5)AF480924External primers
 F: tca ggt gct tca ttg gca gga tca3008–3031
 R: taa aat ttg tac ttt tgg gca ctg ctg3805–3782
Nested primers
 F: tgc aac ctt atg tta gtg cac tcc 3052–3075
 R: tac tgc ctg gtc aac ata tag 3766–3746