Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells
Figure 2
Silibinin suppresses TNF-α- or IFN-γ-induced ICAM-1 protein production in ARPE-19 cells. ARPE-19 cells were cultured to 80% confluence, treated with 25 μM silibinin, 50 μM silibinin, and/or 2 μg/mL tunicamycin in a serum-free medium for 24 h, and stimulated with TNF-α (a) or IFN-γ (b) for a further 24 h. The levels of protein expression were quantified by western blotting using an antibody against ICAM-1 and were expressed as ratios relative to the average values for the 85 kDa protein in the control group. Asterisks () designate responses that are significantly different ().
