Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells
Figure 3
Decreased adhesion of THP-1 cells to ARPE-19 cells after inhibition of ICAM-1 production by silibinin. Confluent ARPE-19 cell cultures were preincubated with 25 μM or 50 μM silibinin for 24 h and subsequently stimulated with TNF-α (a) or IFN-γ (b) for 24 h. Fluorescein-labeled THP-1 cells were added to cytokine treated or untreated monolayers of ARPE-19 cells. The numbers of fluorescein-labeled THP-1 cells were determined. Data from five randomly selected low-power fields for each treatment are presented. Asterisks () designate responses that are significantly different ().
