Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells
Figure 4
Evidence for inhibition of N-glycosylation by silibinin in ARPE-19 cells. (a) ARPE-19 cells at 80% confluence were treated with 30 mM GlcNAc, 25 μM, or 50 μM silibinin for 24 h. The cells were harvested and incubated with fluorescein isothiocyanate (FITC)-LPHA (10 μg/mL), and L-PHA lectin binding was detected by flow cytometry. (b) The ARPE-19 cells were pretreated with 2 μg/mL tunicamycin and/or 50 μM silibinin, treated with or without TNF-α, and treated with or without PNGase F. The levels of protein expression were quantified by western blotting using an antibody against ICAM-1. (c) The ARPE-19 cells were pretreated with 50 μM silibinin and then treated with or without TNF-α. The relative expression of MGAT3 gene was detected by qRT-PCR. Asterisks () designate responses that are significantly different at .
