Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells

Figure 8

Comparison of the effects of GlcN and OGT overexpression on TNF-α-induced ICAM-1 expression. ARPE-19 cells were transfected with pcDNA3.1-OGT and/or pretreated with 50 μM silibinin. The cells were then treated with TNF-α for 24 h in the absence or presence of silibinin or were transfected with pcDNA3.1-OGT. Whole-cell lysates were prepared and analyzed with western blots using antibodies directed against ICAM-1.