The Mouse Median Nerve Experimental Model in Regenerative Research
Figure 2
High-resolution light microscopy. (a)-(b) Representative images of toluidine-blue-stained transverse-sectioned control median nerve (a) and regenerated median nerve 25 days after the crush injury (b). The pictures include two circular frames where the myelinated fibers are counted. The yellow numbers indicate the myelinated fibers that have the top inside the circular frame and therefore are counted. . (c) and (d) illustrate the data obtained with the light microscopic stereological analysis. (c) shows the total number of myelinated fibers in the control group and 25 days after the crush injury, while (d) shows the size parameters (axon diameter, fiber diameter, and myelin thickness) in the two experimental groups. Data are reported as means ± standard deviation of five samples in each group. Statistical analysis was performed using paired t-test (using SPSS 20.0 software). ; ; and .
