Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress

Figure 3

Immunolocalization of peroxiredoxin in E. histolytica strain HM-1 : IMSS during metronidazole stress. The images from left to right in each panel show phase contrast, peroxiredoxin stained by Alexa 488 (green), CaBp-1 stained by Alexa-555, Hoechst 3342 for nuclear staining, and the merged image. Peroxiredoxin was present in the membrane and the nucleus in the untreated cells (panel (1)), during short term metronidazole shock the peroxiredoxin localized to the membrane with loss of nuclear staining within 24 hours (panels (2), (3), and (4)) and then reverted back to the normal position after 48 hours (panel (5)). CaBp1 localized to the cytoplasm. Panels (6) and (7) show untreated cells and cells adapted to metronidazole (20 μM A), respectively. Peroxiredoxin localized to nucleus and membrane in both cases. The fourth panel from left shows fibrillarin stained with Alexa 594 to mark the nucleolus (panels (6) and (7)).