Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress

Table 1

Description of primers used for RT_PCR.

Enzyme	Primer composition	Tm	Amplicon size
Eh peroxiredoxin (Prx)	F 5′ AAA TCA ATT GTG AAG TTA TTG G 3′; R 5′ TCC TAC TCC TCC TTT ACT TTT A 3′	53.6°C; 56.8°C	100 bp
Fe SOD; Accession number; (XM_643735.2)	F 5′ ACA ATT ACC TTA TGC TTA TAA 3′; R 5′ TCC ACA TCC ACA CAT ACA AT 3′	52°C; 54°C	240 bp
Entamoeba histolytica; 18S ribosomal RNA gene	F 5′ TCA GCC TTG TGA CCA TAC TC 3′; R 5′ AAG ACG ATC AGA TAC CGT CG 3′	61.7°C; 68.9°C	200 bp