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BioMed Research International
Volume 2014, Article ID 712312, 8 pages
Research Article

Potent Anti-Inflammatory Activity of Carbohydrate Polymer with Oxide of Zinc

1Immunobiology Laboratory, Mexico’s Juarez Hospital, Ministry of Health, 07760 Mexico City, Mexico
2Biochemistry Department, National Institute of Perinatology, Ministry of Health, 11000 Mexico City, Mexico
3Health Sciences Faculty, Anahuac University, 52786 State of Mexico, Mexico
4Health Research Policies, Coordinating Commission of National Health Institutes and Highly Specialized Hospitals, 01900 Mexico City, Mexico
5Mexico’s Juarez Hospital, Ministry of Health, 07760 Mexico City, Mexico

Received 19 November 2013; Accepted 29 January 2014; Published 12 March 2014

Academic Editor: Iva Dekaris

Copyright © 2014 Mario Adan Moreno-Eutimio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pebisut is a biological adhesive composed of naturally occurring carbohydrates combined with zinc oxide (ZnO) initially used as a coadjutant for healing of anastomoses. Likewise some works demonstrated that carbohydrate complexes exerts anti-inflammatory activity and it is widely known that ZnO modulate inflammation. However, the direct effects of Pebisut on isolated cells and acute inflammatory responses remained to be investigated. The present study evaluated anti-inflammatory effect of Pebisut using lipopolysaccharide (LPS) stimulated human mononuclear cells, chemotaxis, and cell infiltration in vivo in a murine model of peritonitis. Our data show that human cells treated with different dilutions of Pebisut release less IL-6, IL-1β, and IL-8 after LPS stimuli compared with the control treated cells. In addition, Pebisut lacked chemotactic activity in human mononuclear cells but was able to reduce chemotaxis towards CCL2, CCL5, and CXCL12 that are representative mononuclear cells chemoattractants. Finally, in a murine model of peritonitis, we found less number of macrophages (F4/80+) and T lymphocytes (CD3+) in peritoneal lavages from animals treated with Pebisut. Our results suggest that Pebisut has anti-inflammatory activity, which might have a beneficial effect during anastomoses healing or wounds associated with excessive inflammation.