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BioMed Research International
Volume 2014, Article ID 714173, 4 pages
http://dx.doi.org/10.1155/2014/714173
Research Article

Modified PEHPS Medium as an Alternative for the In Vitro Culture of Giardia lamblia

1Laboratorio de Bioquímica y Fisiología Celular, División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano Del Seguro Social, Administración de Correo No. 4, Apartado Postal 020-E Colonia Independencia, 64720 Monterrey, NL, Mexico
2Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Avenida Universidad s/n, Ciudad Universitaria, 66451 San Nicolás de los Garza, NL, Mexico
3Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Avenida Pedro de Alba y Manuel L. Barragán s/n, Ciudad Universitaria, 66451 San Nicolás de los Garza, NL, Mexico
4Coordinador Auxiliar Medico, Instituto Mexicano Del Seguro Social, Delegación Nuevo León, Gregorio Torres de Quevedo No. 1950 Ote, 64000 Monterrey, NL, Mexico

Received 28 February 2014; Revised 28 April 2014; Accepted 13 May 2014; Published 21 May 2014

Academic Editor: Thean Hock Tang

Copyright © 2014 Javier Vargas-Villarreal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. G. Clark and L. S. Diamond, “Methods for cultivation of luminal parasitic protists of clinical importance,” Clinical Microbiology Reviews, vol. 15, no. 3, pp. 329–341, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. M. Limoncu, A. Özbilgin, I. Balcioglu, and Y. Özbel, “Evaluation of three new culture media for the cultivation and isolation of Leishmania parasites,” Journal of Basic Microbiology, vol. 44, no. 3, pp. 197–202, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. R. D. Adam, “Biology of Giardia lamblia,” Clinical Microbiology Reviews, vol. 14, no. 3, pp. 447–475, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. D. E. Katz, D. Heisey-Grove, M. Beach, R. C. Dicker, and B. T. Matyas, “Prolonged outbreak of giardiasis with two modes of transmission,” Epidemiology and Infection, vol. 134, no. 5, pp. 935–941, 2006. View at Publisher · View at Google Scholar · View at Scopus
  5. R. C. A. Thompson, “Giardiasis as a re-emerging infectious disease and its zoonotic potential,” International Journal for Parasitology, vol. 30, no. 12-13, pp. 1259–1267, 2000. View at Publisher · View at Google Scholar · View at Scopus
  6. M. S. Wolfe, “Giardiasis,” Clinical Microbiology Reviews, vol. 5, no. 1, pp. 93–100, 1992. View at Google Scholar · View at Scopus
  7. D. B. Keister, “Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 77, no. 4, pp. 487–488, 1983. View at Google Scholar · View at Scopus
  8. B. D. Mata-Cárdenas and S. Said-Fernández, “Wall synthesis in axenic cultures of Entamoeba histolytica grown in PEHPS medium,” Archivos de Investigacion Medica, vol. 17, supplement 1, pp. 25–30, 1986. View at Google Scholar · View at Scopus
  9. S. Said-Fernandez, J. Vargas-Villarreal, J. Castro-Garza et al., “PEHPS medium: an alternative for axenic cultivation of Entamoeba histolytica and E. invadens,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 82, no. 2, pp. 249–253, 1988. View at Google Scholar · View at Scopus
  10. D. B. Mata-Cárdenas and S. Said-Fernández, “Development of a simplified method for the axenic culture of Entamoeba histolytica,” Archivos de Investigacion Medica, vol. 21, pp. 247–251, 1990. View at Google Scholar
  11. B. D. Mata-Cárdenas, J. Vargas-Villarreal, L. Navarro-Marmolejo, and S. Said-Fernández, “Axenic cultivation of Trichomonas vaginalis in a serum-free medium,” Journal of Parasitology, vol. 84, no. 3, pp. 638–639, 1998. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Said-Fernández, E. Campos-Góngora, F. González-Salazar, H. G. Martinez-Rodriguez, J. Vargas-Villarreal, and J. M. Viader-Salvadó, “Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material,” Journal of Parasitology, vol. 87, no. 4, pp. 919–923, 2001. View at Google Scholar · View at Scopus
  13. B. D. Mata-Cardenas, J. Vargas-Villarreal, H. G. Martinez-Rodriguez, and S. Said-Fernandez, “Entamoeba histolytica axenic growth improvement by ox bile,” Archives of Medical Research, vol. 26, no. 4, pp. 441–444, 1995. View at Google Scholar · View at Scopus
  14. R. Cedillo-Rivera, J. A. Enciso-Moreno, A. Martínez-Palomo, and G. Ortega Pierres, “Isolation and axenization of Giardia lamblia isolates from symptomatic and asymptomatic patients in Mexico,” Archivos de Investigacion Medica, vol. 22, no. 1, pp. 79–85, 1991. View at Google Scholar · View at Scopus
  15. J. Castro-Garza, F. Anaya-Velazquez, S. Said-Fernandez, and M. T. Gonzalez Garza, “Comparable growth of a Trichomonas vaginalis strain in PEHPS and TYI-S-33 media,” Archives of Medical Research, vol. 27, pp. 567–569, 1996. View at Google Scholar
  16. D. Bansal, H. S. Bhatti, and R. Sehgal, “Role of cholesterol in parasitic infections,” Lipids in Health and Disease, vol. 4, article10, 2005. View at Publisher · View at Google Scholar · View at Scopus
  17. H. D. Lujan and T. E. Nash, “The uptake and metabolism of cysteine by Giardia lamblia trophozoites,” Journal of Eukaryotic Microbiology, vol. 41, no. 2, pp. 169–175, 1994. View at Google Scholar · View at Scopus
  18. F. Li, N. Vijayasankaran, A. Shen, R. Kiss, and A. Amanullah, “Cell culture processes for monoclonal antibody production,” mAbs, vol. 2, no. 5, pp. 466–479, 2010. View at Publisher · View at Google Scholar · View at Scopus