Modified PEHPS Medium as an Alternative for the In Vitro Culture of Giardia lamblia

Table 1

Comparative ingredients of original PEHPS media [9] with modified PEHPS media.

Compound	Brand	PEHPS [9]	Modified PEHPS
EHP	Homemade [9]	250 mL	250 mL
Casein peptone	BD Bioxon	10 g/L	10 g/L
Ascorbic acid	Sigma Aldrich	0.2 g/L	1 g/L
Cysteine	Sigma Aldrich	1.0 g/L	1.26 g/L
Glucose	Tecnica Quimica	6 g/L	6 g/L
K2HPO4	J T Baker	1	1
KH2PO4	Monterrey	0.6	0.6
Bovine bile	Sigma Aldrich	0.0 g/L	0.6 g/L
Deionized water		Enough to fill a 1/L	Enough to fill a 1/L

EHP: pancreas-liver extract.