mAb CZP-315.D9: An Antirecombinant Cruzipain Monoclonal Antibody That Specifically Labels the Reservosomes of Trypanosoma cruzi Epimastigotes
Figure 2
Immunolocalization of cruzipain and its colocalization with TcHIP/AC and ingested transferrin in Trypanosoma cruzi epimastigotes. The nucleus (arrowhead) and kinetoplast (arrow) are stained blue with Hoechst 33342. (a) Incubation with preimmune serum. (b) Incubation with anti-TcCruzipain serum. Note labeling of reservosomes and a single spot at the anterior end of the cell. (c) Incubation with CZP-315 hybridoma supernatant. Note that only reservosomes are labeled. (d) Incubation with CZP-315.D9 monoclonal antibody. Labeling is specific for reservosomes. ((e)–(h)) Colocalization of anti-TcCruzipain serum (green staining) with the Golgi marker TcHIP (red staining) in TcHIP/AC-transfected epimastigotes. ((i)-(l)) Endocytosis assay with transferrin coupled to Alexa 633 (red staining), and its colocalization with cruzipain (green staining) in epimastigotes, resulting in yellow staining. Bars = 5 μm.
