mAb CZP-315.D9: An Antirecombinant Cruzipain Monoclonal Antibody That Specifically Labels the Reservosomes of Trypanosoma cruzi Epimastigotes
Figure 3
Immunolocalization of cruzipain in Trypanosoma cruzi epimastigotes by transmission electron microscopy. Ultrathin sections were incubated with the mAb CZP-315.D9, followed by a secondary antibody coupled to 10 nm gold particles. Note the specific gold labeling (arrows) in the reservosomes. Weaker labeling was found in cells embedded with Lowicryl K4M resin ((a)–(c)), while more intense labeling was found in the electrondense reservosomes of cells embedded with Lowicryl K4M MonoStep resin ((d)-(e)). (e) shows a high magnification of the area delimited in (d). N: nucleus; R: reservosome.
