Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena
Figure 3
Experiment III. Effect of NE on melatonin secretion from the ovine pineal explants treated with transcription and translation inhibitors. The ovine pineal glands () were divided into four explants, which were randomly assigned to the experimental groups. The explants were incubated in the medium containing group I, 0.5% DMSO from 301 min to 360 min and 10 μM NE + 0.5% DMSO from 361 min to 720 min of the experiment; group II, 10 μM ACT D from 301 min to 360 min and 10 μM NE + 10 μM ACT D from 361 min to 720 min of the experiment; group III, 100 μM CLX from 301 min to 360 min and 10 μM NE + 100 μM CLX from 361 min to 720 min of the experiment; and group IV, 100 μM ANS from 301 min to 360 min and 10 μM NE + 100 μM ANS from 361 min to 720 min of the experiment. The mean melatonin secretion between 241 min and 300 min of the experiment was taken as 100%. The data presented are means. The values significantly different () between groups were signed with different letters. Blue bar is period of incubation in the medium with inhibitors; red bar is period of incubation in the medium with NE.