Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena

Table 1

Inhibition of protein synthesis in ovine and rat pineal explants by cycloheximide and anisomycin. Radioactivity of the protein fraction of the pineal explants incubated in the medium containing 0.5% DMSO (control), 100 μM CLX, or 10 μM ANS for 60 minutes and then in the medium containing vehiculum or the inhibitors as well as 10 μM norepinephrine and ³H-leucine for 120 minutes. The assay was performed as described in Material and Methods.

Treatment	^3H-leucine incorporation into proteins
	Ovine pineal explants		Rat pineal explants
	×10^6 dpm/mg protein; (mean ± SEM, 𝑛=6)	%	×10^6 dpm/mg protein; (mean ± SEM, 𝑛=6)	%
Control	22.24±1.21	100.00	19.41±0.81	100.00
CLX 100 𝜇M	0.43±0.02	1.92	0.41±0.07	2.11
ANS 10 𝜇M	0.72±0.08	3.21	0.83±0.09	4.22