Research Article
High Resolution Melting Analysis for Rapid Mutation Screening in Gyrase and Topoisomerase IV Genes in Quinolone-Resistant Salmonella enterica
Table 2
PCR primer sequences for HRM analysis of gyrase and topoisomerase IV genes.
| | Primer | Sequence (5′-3′)b | Amplicon size (bp) |
| | gyrA-F | CAATGACTGGAACAAAGCCTA | 164 | | gyrA-R | AACCGAAGTTACCCTGACCA | | gyrB-F | TGTCCGAACTGTACCTGGTG | 198 | | gyrB-R | ACTCGTCGCGACCGATAC | | parC-F | CGTCTATGCGATGTCAGAGC | 219 | | parC-R | ATCGCCGCGAATGACTTC | | parE-F | TACCGCGCAGGATCTTAATC | 193 | | parE-R | GATCGCCACGGAAATATCAT |
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Primers designed in this study.
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