Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter
Figure 4
Anti-HBV pri-miR expression in livers of HBV transgenic mice following treatment with HD Ads expressing artificial mono- or trimeric pri-miRs under control of the CMV or MTTR promoters. Northern blot analyses of 30 μg (a) and 60 μg (b) of RNA isolated from mouse livers 1 week after infection with HD Ads. Blots hybridized with probes complementary to predicted guides 5, 8, or 9 were stripped and reprobed for U6 snRNA to confirm equal loading. (c) Quantification of indicated anti-HBV guide sequences generated from CMV and MTTR promoters was determined using a phosphorimager. Data were normalized to U6 snRNA expression and a representative example is shown.
