Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter
Figure 6
Intrahepatic HD Ad copy numbers and alanine transaminase activity following HD Ad administration to HBV transgenic mice. (a) Mice were injected intravenously with saline or a single dose of 5 × 109 infectious viral particles. DNA isolated from mice livers harvested at 48 hours, 1 week, and 3 and 6 weeks after injection was subjected to Q-PCR analysis to determine viral particle equivalents (VPEs). Data are expressed as means (±SEM) from three mice. Statistically significant differences were determined by comparisons to values obtained at 2 days. Analyses were carried out using a pair-wise Student’s -test. values less than 0.05 () or less than 0.01 () were considered statistically significant. (b) Histochemical detection of Beta-galactosidase activity in livers of mice treated with saline or HD Ad Δ28. Frozen liver sections were stained for reporter gene activity at 48 hours, 1 week, 3 weeks, and 6 weeks after saline or HD Ad Δ28 administration. (c) Serum ALT activity in HD Ad infected mice. Serum activity of ALT was measured 48 hours following injection with saline or 5 × 109 infectious particles of the indicated HD As. Data is expressed as means (±SEM) from groups of six mice. The statistically significant differences were calculated as indicated above.
