Perinatal Hypoxia-Ischemia Reduces α7 Nicotinic Receptor Expression and Selective α7 Nicotinic Receptor Stimulation Suppresses Inflammation and Promotes Microglial Mox Phenotype
Figure 4
α7 receptor agonist AR-R 17779 polarize microglia towards Mox phenotype. Primary microglia cultures were stimulated with LPS (10 ng/mL) with or without α7R agonist AR-R 17779 (10 µM) for 4 h. Cells were collected and gene expression of (a) M1 markers CD86 and iNOS, (b) M2 markers CD206 and Arginase 1 (Arg1) and (c) Mox markers heme oxygenase (Hmox1) and sulfiredoxin-1 (Srxn1) was investigated. Treatment with LPS and α7R agonist AR-R 17779 did not influence microglial expression of M1 markers and M2 marker CD206; however, M2 marker Arg1 was downregulated by LPS, and upregulated by the combination of LPS and α7R agonist AR-R 17779 ((a)-(b)). Mox markers Hmox1 and Srxn1 were both upregulated by LPS and α7R agonist AR-R 17779 treatment (c). Graph represents pooled data from 4-5 independent experiments, /group for all except Control AR-R 17779; . Gene expression was normalized to YWHAZ and analyzed using CT method. Data are expressed as mean ± SEM, , ,.
