Vascular Endothelial Growth Factor Increases during Blood-Brain Barrier-Enhanced Permeability Caused by Phoneutria nigriventer Spider Venom
Figure 3
Photomicrographs of cerebellar cortex of rats aged 14 days 5 hours after administration of saline solution ((a), (c), and (e)) or Phoneutria nigriventer venom (PNV) ((b), (d), and (f)). PNV increased VEGF (b), Flt-1 (d), and Flk-1 (f) labeling. ML = molecular layer; PC = Purkinje cells, and GL = granular layer. Arrows point Purkinje cells positive for VEGF, Flt-1, and Flk-1; triangles show nonuniformly scattered labeled nuclei inside the molecular layer. Bars = 25 μm for all panels. Inserts illustrate the expression of Flt-1 receptor in the endothelium nuclei, whereas Flk-1 receptor is expressed in nuclei and cytoplasm of endothelial cells of envenomed rats (Bars = 10 μm (d) and 25 μm (f)).
