BioMed Research International / 2014 / Article / Fig 4

Research Article

The Effects of Acute Stress-Induced Sleep Disturbance on Acoustic Trauma-Induced Tinnitus in Rats

Figure 4

Orexin immunoreactivity in the hypothalamus. (a) A photograph showing orexin immunostaining located in the hypothalamus under a low magnification (scale bar: 1000 µm). Inserted picture showing the presence of orexin-positive staining in the cytoplasm and the processes of the neurons (scale bar: 100 µm). (b) Estimated total number of orexin-positive neurons in the ipsilateral and contralateral hypothalamus in sham, exposed-clean, and exposed-dirty animals. Bars represent means ± 1 SEM.