Research Article
Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro
Table 5
Experimental design and results of the CCD.
| Run | Type | Moisture | Beef extract | NaH2PO4 | Response () (units/mL) |
| 1 | Factorial | −1 (60) | −1 (0.1) | 1 (0.05) | 2175 | 2 | Center | 0 (80) | 0 (0.2) | 0 (0.0375) | 2553 | 3 | Center | 0 (80) | 0 (0.2) | 0 (0.0375) | 3150 | 4 | Center | 0 (80) | 0 (0.2) | 0 (0.0375) | 2868 | 5 | Factorial | −1 (60) | 1 (0.3) | 1 (0.05) | 2592 | 6 | Factorial | 1 (100) | −1 (0.1) | 1 (0.05) | 2916 | 7 | Axial | 1.682 (113.64) | 0 (0.2) | 0 (0.0375) | 1522 | 8 | Axial | 0 (80) | 0 (0.2) | −1.662 (0.0182) | 2731 | 9 | Factorial | 1 (100) | 1 (0.3) | 1 (0.05) | 3699 | 10 | Axial | 0 (80) | −1.682 (0.34) | 0 (0.0375) | 2463 | 11 | Center | 0 (80) | 0 (0.2) | 0 (0.0375) | 3147 | 12 | Factorial | −1 (60) | −1 (0.1) | −1 (0.025) | 1372 | 13 | Factorial | −1 (60) | 1 (0.3) | −1 (0.025) | 2689 | 14 | Axial | 0 (80) | −1.682 (0.06) | 0 (0.0375) | 3012 | 15 | Factorial | 1 (100) | 1 (0.3) | −1 (0.025) | 2182 | 16 | Factorial | 1 (100) | −1 (0.1) | −1 (0.025) | 2209 | 17 | Center | 0 (80) | 0 (0.2) | 0 (0.0375) | 3048 | 18 | Axial | 0 (80) | 0 (0.2) | 1.682 (0.0568) | 3334 | 19 | Center | 0 (80) | 0 (0.2) | 0 (0.0375) | 2779 | 20 | Axial | −1.682 (46.36) | 0 (0.2) | 0 (0.0375) | 86 |
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