BioMed Research International / 2014 / Article / Fig 4

Research Article

Effect of Chlorogenic Acid (5-Caffeoylquinic Acid) Isolated from Baccharis oxyodonta on the Structure and Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus terrificus

Figure 4

(a) Enzymatic activity was analysed using 4N3OBA as a substrate and monitored at a wavelength of 425 nm. sPLA2 : 5CQA shows a significant decrease compared with native sPLA2. (b) The effect of the substrate on the enzymatic activity of the native and 5CQA-pretreated sPLA2 (sPLA2 : 5CQA). Chemical treatment of sPLA2 with 5CQA shifts both the Km and Vmax of the native sPLA2. (c) The effect of native sPLA2 and sPLA2 : 5CQA against Clavibacter michiganensis pv michiganensis (gram-positive bacteria). Error bars indicate the SEM; compared with the saline control.

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