BioMed Research International / 2014 / Article / Fig 7

Research Article

Effect of Chlorogenic Acid (5-Caffeoylquinic Acid) Isolated from Baccharis oxyodonta on the Structure and Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus terrificus

Figure 7

The results of the pharmacological assays. (a) Paw oedema induced after the injection of sPLA2 and sPLA2 : 5CQA (10 μg/paw) into the right paw of Swiss mice. Measurements were performed after 30, 60, 120, 180, and 240 min, and no differences were observed after the treatment with 5CQA. (b) Myonecrosis was assayed based on the creatine kinase levels in Swiss mice. The right gastrocnemius muscle was injected with 50 μL of a 0.5 mg/mL sPLA2 sample (native or 5CQA-treated). Control mice received an equal volume of 0.15 M NaCl. After 3 h, the mice were anaesthetised, and blood was collected from the abdominal vena cava into tubes containing heparin as an anticoagulant. The results are expressed as the units of enzymatic activity per litre (U/L). Error bars indicate the SEM. compared with sPLA2 activity.

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