Hyaluronan-Phosphatidylethanolamine Polymers Form Pericellular Coats on Keratinocytes and Promote Basal Keratinocyte Proliferation
Figure 3
Topical application of HA-PE stimulates HA accumulation in wild type and CD44−/− keratinocytes in mouse skin in vivo. Topical application of HA-PE to shaved B57/BL6 wild type mice increases HA in the keratinocyte layer as detected by biotinylated HABP (blue staining indicates hyaluronan detected by HABP probe). (a) Staining 3 days after application is not detectably different in the dermis and although patchy and variable in the epidermis is significantly different in HA-PE versus vehicle controls (brown staining indicates hyaluronan detected by HABP probe) (). Images were taken with a 20X bright field objective. Similar results were obtained when the epidermal layer was analyzed for hyaluronan after HA-PE was applied to B57/BL6 CD44−/− mice. Significantly greater HA staining was observed in HA-PE treated versus vehicle controls (). (b) Images were taken with a 10X brightfield objective. Values in (a) and (b) are the Mean and S.E.M mice, 5 tissue section/mouse.
