Amarogentin, a Secoiridoid Glycoside, Abrogates Platelet Activation through PLCγ2-PKC and MAPK Pathways
Figure 1
Amarogentin inhibits collagen-induced platelet aggregation and ATP release. Washed platelets (3.6 × 108 cells/mL) were incubated with solvent control (0.5% DMSO) or amarogentin (15 μM~120 μM) for 3 min in an aggregometer cuvette. Then, 1 μg/mL collagen (a), 0.01 IU/mL thrombin (b), 60 μM arachidonic acid (AA) (c), or 1 μM U46619 (d) was added to induce platelet aggregation and ATP-release ((a), upper tracings) for 6 min. Profiles ((a)–(d)) are representative of 3 independent experiments.
