The Interrelationship between Leukotriene B4 and Leukotriene-A4-Hydrolase in Collagen/Adjuvant-Induced Arthritis in Rats

Table 2

LTA4H activity (U) in the plasma, synovial fluid (SF), soluble (SO), and solubilized membrane-bound (MB) fractions of synovial tissue (ST) and peripheral blood mononuclear cells (PBMCs) from control, CIA-arthritic, and CIA-resistant rats.

Samples	Control	CIA-arthritic	CIA-resistant	ANOVA
PLASMA	20.89±0.77	28.92±3.33	29.24±3.77	𝑃=0.1540
SF	12.66±0.86^a	21.16±1.34^b	44.91±3.00^c	𝑃<0.0001
SO-ST	57.72±8.20	38.81±6.83	52.43±4.61	𝑃=0.2023
MB-ST	13.85±0.03^a	0±3.45^a	56.29±15.10^b	𝑃=0.0060
SO-PBMCs	0±12.48^a	21.76±10.01^b	753.72±5.23^c	𝑃<0.0001
MB-PBMCs	142.30±31.50^a	57.42±14.53^b	48.86±0.62^b	𝑃=0.0306

Values ​ ​are mean ± SEM. U = LTA4H unit = LTA4H activity in 1 mL sample that catalyzes the formation of 1 picomole of LTB4 per minute. Number of animals=3, values ​ ​of each animal measured in duplicate. Student-Newman-Keuls, 𝑃<0.05: comparison among control versus CIA-arthritic versus CIA-resistant in the same row (different letters indicate significant difference).