Comparative Study of Corneal Endothelial Cell Damage after Femtosecond Laser Assisted Deep Stromal Dissection
Figure 3
Alizarin Red and Hoechst 33342 staining of the endothelium after femtosecond laser cutting. (a) The endothelial cell borders of corneal buttons in the 50 μm group were not clear, and in some place the endothelial cells were completely lost. (b) Hoechst 33342 staining showed that the nucleus was uneven and shrinking, with many apoptotic cells present in the 50 μm group. ((c) and (e)) The endothelial cell borders of the 150 μm group and the control group were clear and had a normal hexagonal structure. ((d) and (f)) No apoptotic cells could be detected in the 150 μm group or the control group.
