Detection Progress of Selected Drugs in TLC

Table 5

Characteristics of densitometric bands of β-sitosterol after dipping and spraying with the use of particular visualizing reagents [47].

	10 µg of 𝛽-sitosterol				5 µg of 𝛽-sitosterol
	Area (AU)	Height (AU)	Angle α (°)	𝜆max (nm)	Area (AU)	Height (AU)	Angle α (°)	𝜆max (nm)
Without using visualizing reagent	4797	144.5	15	200	3243	82.6	10	200
Rhodamine B
Dipping	646	34	110	200	112	9.4	21	595
Spraying	3807	115.8	12	200	1770	59.6	22.5	584
Gentian violet
Dipping	7161	312.3	45	510	1418	47.8	5	517
Spraying	5148	146.9	9.5	200	4354	112.4	19	606
Methylene violet
Dipping	3017	110	19	200	1490	49	9.5	200
Spraying	7561	213.9	8.5	200	4056	126.3	10	200
Methyl green
Dipping	1415	53.3	105	200	538	24.8	47	473
Spraying	5966	167.4	9	200	4560	103.5	16	200
Malachite green
Dipping	2235	82.7	51	640	966	40.7	42	643
Spraying	5338	155.5	8	643	3444	111.4	10	649
Janus blue
Dipping	1029	49	134	678	53	6.2	41.5	685
Spraying	2474	69.1	24	667	1772	50.1	30	662