Baicalein Induces Apoptosis and Autophagy via Endoplasmic Reticulum Stress in Hepatocellular Carcinoma Cells
Figure 6
Diverse roles of UPR proteins in baicalein-induced apoptosis.(a) SMMC-7721 cells were transfected with scrambled RNA (NC) or CHOP-targeting siRNA (si-CHOP) for 48 h and treated with 0, 100, and 200 μM baicalein for 24 h. Protein levels of cleaved PARP and CHOP were determined by western blotting. (b) SMMC-7721 cells were transfected with scrambled RNA (NC) or eIF2α-targeting siRNA (si-eIF2α) and then treated with 0, 100, and 200 μM baicalein for 24 h. Protein levels of cleaved PARP phosphorylated eIF2α and eIF2α were determined. (c) After being transfected with scrambled RNA (NC) or IRE1α-targeting siRNA (si-IRE1α), SMMC-7721 cells were treated with the indicated dose of baicalein for 24 h and subjected to western blotting to analyze the level of cleaved PARP, IRE1α, phosphorylated JNK, and total JNK. GAPDH served as a loading control.
