Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling

Table 1

Binding and thermodynamic parameters for the patulin-HSA interaction at different temperatures in Trisbuffer (pH = 7.4).

T/K	Equation (2)		Equation (3)		n	Δ𝐻^0/kJmol^−1	Δ𝑆^0/J mol^−1	Δ𝐺^0/kJ mol^−1
	𝐾SV/M^−1	R	𝐾𝐴/M^−1	R
288	1.17×10^4	0.9961	2.60×10^4	0.9769	1.10			−22.46
300	1.40×10^4	0.9985	4.59×10^4	0.9914	1.17	8.06	106.0	−23.73
310	1.48×10^4	0.9972	7.01×10^4	0.9982	1.34			−24.79