Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells
Figure 6
The effect of PMCAs downregulation on bioenergetic parameters. (a) The representative traces of O2 consumption. 2 mM KCN was used to assess minimal O2 consumption and 1 μM FCCP to measure uncoupled respiration. (b) Quantification of respiratory rate. The respiration rate in control cells was taken as 100% (dotted line). versus control line; maximal or minimal O2 consumption versus resting. (c) Mean time course of NAD(P)H autofluorescence. 2 mM KCN and 1 μM FCCP were used to obtain maximal and minimal signals, respectively. AU: arbitrary units. (d) Quantification of NAD(P)H changes. The autofluorescence level in control cells was taken as 100% and is indicated as a dotted line in the graph. versus control line; maximal or minimal signal versus resting. : mock-transfected PC12, _2: PMCA2-reduced PC12, and _3: PMCA3-reduced PC12.
