Chondrogenic Differentiation of Human Adipose-Derived Stem Cells: A New Path in Articular Cartilage Defect Management?

Table 1

Media for the chondrogenic differentiation of ASCs.

	Basal medium	Supplements
(1)	DMEM + 1% FCS	(i) TGF-𝛽1 (10 ng/mL)
		(ii) ASAP (50 nM)
		(iii) Insulin (6.25 𝜇g/mL)
(2)	DMEM	(i) TGF-𝛽3
		(ii) Albumin (1.25 𝜇g/mL)
		(iii) Dexamethasone (10–7 M)
		(iv) Ascorbic acid
		(v) Transferrin (6.25 𝜇g/mL)
		(vi) Insulin (6.25 𝜇g/mL)
(3)	OriCell	(i) TGF-𝛽3
		(ii) Dexamethasone
		(iii) Ascorbic acid
		(iv) ITS cell culture supplement
		(v) Sodium pyruvate
		(vi) Proline

According to (1) Zuk et al., [10] (2) Baptista et al., [11] (3) OriCell (Cyagen, GUXMX-90041, Santa Clara, CA, USA).
ASAP: ascorbate-2-phosphate; DMEM: Dulbecco’s modified Eagle’s medium; FCS: fetal calf serum; TGF: transforming growth factor.