High Guanidinium Permeability Reveals Dehydration-Dependent Ion Selectivity in the Plasmodial Surface Anion Channel
Figure 2
Gdm+ uptake is primarily via PSAC. (a and b) Osmotic lysis kinetics for Gdm+ uptake into Dd2 and HB3 at 15°C. Traces reflect inhibition dose responses using 0, 0.024, 0.12, 0.6, 3.0, and 15 μM ISPA-28 (top to bottom, resp., in each panel). While inhibition is clear with Dd2-infected cells, there is negligible effect with HB3-infected cells. (c) Symbols represent mean ± S.E.M. of tallied dose responses from experiments as in panels (a) and (b) for Gdm-Cl and sorbitol (white and black symbols, resp.) using Dd2- and HB3-infected cells (circles and triangles, resp.). The Gdm+ and sorbitol dose responses do not differ ( trials at each concentration, for comparisons between solutes in each parasite.).
