Differential Expression of Immunogenic Proteins on Virulent Mycobacterium tuberculosis Clinical Isolates
Proteomics to identify differential proteins between LAM10406 and H12425 M. tuberculosis strains. (a) Equal amounts of proteins from culture supernatants of Mtb LAM10406 and H12425 were resolved on 1D 12% polyacrylamide gels, which were stained with colloidal Coomassie. The identified differential protein is indicated with the arrow. (b) Differential proteins identified by 2D gel electrophoresis. The first-dimension isoelectric focusing used a pH range of 4–7. The second-dimension SDS-PAGE was on 12% polyacrylamide gels, which were silver stained. Differential proteins present in the cell wall and cell-associated fractions of the Mtb LAM10406 and H12425 strains are indicated.