Differential Expression of Immunogenic Proteins on Virulent Mycobacterium tuberculosis Clinical Isolates
Figure 1
Proteomics to identify differential proteins between LAM10406 and H12425 M. tuberculosis strains. (a) Equal amounts of proteins from culture supernatants of Mtb LAM10406 and H12425 were resolved on 1D 12% polyacrylamide gels, which were stained with colloidal Coomassie. The identified differential protein is indicated with the arrow. (b) Differential proteins identified by 2D gel electrophoresis. The first-dimension isoelectric focusing used a pH range of 4–7. The second-dimension SDS-PAGE was on 12% polyacrylamide gels, which were silver stained. Differential proteins present in the cell wall and cell-associated fractions of the Mtb LAM10406 and H12425 strains are indicated.
