Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-Ras/HMGA2/Snail Axis in Hepatocellular Carcinoma

Figure 6

Reexpression of let-7g induced cell apoptosis and changes of cell cycle. (a) Qualification assay of cell apoptosis of MHCC97-H and HCCLM3 after reexpression of let-7g. (b) Quantitation assay of cell apoptosis of MHCC97-H and HCCLM3 after re-expression of let-7g (**𝑃<0.01, Student’s 𝑡-test). (c) Qualification assay of cell cycle of MHCC97-H and HCCLM3 after reexpression of let-7g. (d) Quantitation assay of cell cycle of MHCC97-H and HCCLM3 after reexpression of let-7g (**𝑃<0.01, Student’s 𝑡-test). (e) Biochemical analysis of proteins that are reported to be involved in the regulation of cell cycle. The representative results out of three times experiments were shown here. 80 μg total protein was loaded per lane when performing Western-blotting.

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