BioMed Research International / 2014 / Article / Fig 4

Research Article

Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

Figure 4

Immunoreactivity assessment of extract components of burn wounds with antibodies against FN. The extract components after electrophoretic separations in gradient polyacrylamide gel 4–15% were subjected to electrotransfer on the membrane Immobilon P and next to the reaction with the antibodies by Western blotting method; lane 1, molecular mass markers, lanes 2, 3, 4, and 5, components released on the 5th day of healing from wounds treated with NaCl, SSD, propolis, and propolis vehicle. The arrows indicate the migration position of molecules of known molecular masses, while the stars indicate the position of fibronectin monomers.

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