Evolutionary Dynamics of rDNA Clusters in Chromosomes of Five Clam Species Belonging to the Family Veneridae (Mollusca, Bivalvia)
Two-color FISH of telomeric (TEL, fluorescein, green) and 5S or major rDNA (5S or ITS, digoxigenin, Rhodamine, red) probes to surface spread synaptonemal complexes of Venerupis corrugata (VCO (a)), Ruditapes decussatus (RDE (b)), Ruditapes philippinarum (RPH (c)), and Venus verrucosa (VVE (d)) counterstained with DAPI. Note that all telomeric signals appear as tightly packed single or double green dots attached to the SCs at both ends of each bivalent in the four clam species. On the other hand, the subterminal red signals corresponding to 5S (VCO (a); RDE (b)) and ITS (RPH (c), VVE (d)) rDNA probes surround the telomeric signals radiating away from the SCs in all clams. Scale bars: 5 μm.
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