Microparticles: A New Perspective in Central Nervous System Disorders
Figure 3
Identification of microparticles by flow cytometry based on particle size and surface protein expression. (a) Traceable beads of a defined size, in this case 900 nm (Nanobead NIST Traceable Particle Size Standard, Polysciences Inc., Warrington, PA, USA), were used to define microparticles in the P1 gate. (b) Endothelial microparticles are identified in platelet poor plasma as CD31+/CD42b− events in the upper left quadrant within this P1 gate. Methods are based on those described by Jenkins et al. [60].
